Product Description

The presence of certain bacteria can quickly transform relatively innocuous sulphur compounds into highly toxic, corrosive and reactive sulphides. The Sig Sulphide test kit allows you to detect the presence of these SRB – and avoid the build-up of sulphides in industrial fluids and lubricants, as well as risk of Microbially Influenced Corrosion (MIC) in bilge, ballast waters, closed system water and metal working fluid systems.

For product specific documents such as Product Instructions and Material Safety Data Sheets, simply click on the Product Downloads text below.

Sig Sulphide Downloads



The build-up of sulphides and the corrosion they cause can wreak havoc and Sig Sulphide™ is a fast, effective way of early detection – thereby avoiding the
related costs and consequences.


Anyone can easily use the kit, by adding a small amount of the fluid to the screw-capped tube of colour-changing testing gel.


Delivering easy-to-read results, the test gives a clear indication of the severity of an SRB infection, and the associated corrosion risks. Sig Sulphide™ also shows good correlation with the NACE TMO 194 standard test method.


The test is much faster and many times less expensive than the equivalent laboratory tests for detecting SRB.

Trafalgar Scienific, UK:

ECHA is always very, very prompt and efficient.

What is the test?

The test consists of a screw-capped, glass tube half filled with a selective microbiological culture gel that semi-quantitatively indicates the presence of sulphide generating bacteria by the rate and extent of the development of a black colour. The sample is added to the tube which is kept warm (incubated) for up to seven days; results are examined at regular intervals.

What is the test used for?

The Sig Sulphide™ test is used to detect the presence of microorganisms that can generate corrosive sulphide, e.g. Sulphide Generating Bacteria (SGB) including Sulphate Reducing Bacteria (SRB).

What can I test with the MicrobMonitor Sig Sulphide Test?
  • Closed Water Heating and Cooling Systems
  • Bilge Water
  • Ballast Water
  • Fuel Tank Water Bottom
  • Metal Working Fluids
  • Process Waters, including coolants
  • Water associated with Lubricants
  • Environmental Waters
  • Soil, mud and drill core samples.
  • Metal surfaces which are prone to attack by SRB. A sterile swab can be used to swab surfaces or corrosion pits then thrust into a Sig Sulphide tube
What are the advantages?
  • Quick, easy and safe to use
  • Heavy infection is detected overnight. Much faster than the standard laboratory test method NACE TMO194-94
  • Simple to read results
  • Results are semi quantitative and give an indication of the severity of an infection
  • Many times cheaper than a laboratory test for sulphide generating organisms.
Who can use the test?

Full instructions are supplied and no special training is required to use the Sig Sulphide™ test.

What Pack Sizes are available?

The Sig Sulphide™ test is sold in packs of 10 tests.  If you require a larger pack size for your application please let us know.

What is the Shelf-life of the test?

The Sig Sulphide™ test is manufactured with 1 years Shelf-life from date of production.  We normally supply tests with at least 8 months remaining, please let us know at the time of your order if you require a longer Shelf-life and we will do our best to accommodate you.

What are the storage requirements of the test?

The test can be stored for at least a year at ambient temperature. If the appearance of the test has not changed after prolonged storage it should be suitable for use.


Background Information

Metal working fluids, process waters, bilge waters, ballast waters, lubricants, paper pulp, detergents, fuels, crude oil and sea water can all contain sulphur in varying forms such as sulphate, sulphite, sulphonate, sulphurised oil and mercaptans. These can all be converted to sulphide by consortia of micro-organisms containing both aerobic and anaerobic bacteria. The anaerobic bacteria in the consortia are usually referred to as Sulphate Reducing Bacteria (SRB).

Biochemical reduction of these sulphur compounds often leads to hydrogen sulphide gas being produced by SRB as an end product. Hydrogen sulphide gas is not only an unpleasant smelling gas and skin irritant but it is more toxic than hydrogen cyanide and can kill.

Hydrogen sulphide stains and corrodes ferrous and non-ferrous metals, stone and concrete and it discolours cutting fluids and fuel tank water bottoms. Sulphide generating bacteria cause rapid pitting corrosion of steel in contact with process water, ships’ bilge water, sludges, crude oil and fuel tank water bottoms, detergent washes, and various chemical solutions and slurries. Pitting corrosion of steel piling is often due to SRB activity.  Sulphide production from emulsifying components (eg petroleum sulphonates) leads to emulsion instability.

What about support?

We provide full technical support to all of our customers, regardless of the product or service they are using. Product support via e-mail and phone is completely free of charge to all of our clients, and that is something we are proud to provide.

What is the HS code of the Sig Sulphide Test?

The Harmonized Commodity Description and Coding System generally referred to as “Harmonized System” or simply “HS” is a multipurpose international product nomenclature developed by the World Customs Organization (WCO).

The Sig Sulphide Test is classified as:  Prepared culture media for the development or maintenance of micro-organisms  HS: for export and HS for import.